Skip to main content
Journal ArticleOPEN ACCESS

A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines

  • Kearney J
  • Radbruch A
  • Liesegang B
  • et al.
The Journal of Immunology (1979) 123(4) 1548-1550
DOI: 10.4049/jimmunol.123.4.1548
1.4kCitations
Citations of this article
99Readers
Mendeley users who have this article in their library.

Abstract

We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.

Cite

CITATION STYLE

APA

Kearney, J. F., Radbruch, A., Liesegang, B., & Rajewsky, K. (1979). A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines. The Journal of Immunology, 123(4), 1548–1550. https://doi.org/10.4049/jimmunol.123.4.1548

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free