We have developed a method for the generation of conditional knockouts in Caenorhabditis elegans by expressing transcription activator-like effector nucleases (TALENs) in somatic cells. Using germline transformation with plasmids encoding TALENs under the control of an inducible or tissue-specific promoter, we observed effective gene modifications and resulting phenotypes in specific developmental stages and tissues. We further used this method to bypass the embryonic requirement of cor-1, which encodes the homolog of human severe combined immunodeficiency (SCID) protein coronin, and we determined its essential role in cell migration in larval Q-cell lineages. Our results show that TALENs expressed in the somatic cells of model organisms provide a versatile tool for functional genomics. © 2013 Nature America, Inc. All rights reserved.
CITATION STYLE
Cheng, Z., Yi, P., Wang, X., Chai, Y., Feng, G., Yang, Y., … Ou, G. (2013). Conditional targeted genome editing using somatically expressed TALENs in C. elegans. Nature Biotechnology, 31(10), 934–937. https://doi.org/10.1038/nbt.2674
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