Effects of feeding enriched artemia fransiscana with HUFA, vitamin C and E on growth performance, survival and stress resistance of yellowfin seabream larvae

Abstract

Effects of feeding highly unsaturated fatty acid (HUFA), vitamins E and C enriched Artemia fransiscana nauplii on growth, survival and stress resistance of yellowfin seabream, A. latus were investigated. Larvae at first exogenous feeding with 53 ± 5 mg body weight were fed with HUFA+5%, 10% and 15% vitamin C-enriched Artemiai (C1, C2 and C3 groups, respectively), HUFA+5% and 10% vitamin E-enriched Artemia (E1 and E2 groups, respectively), HUFA+2.5% (W/W) vitamin C and E enriched Artemia (CE2 group), HUFA+5% (W/W) vitamin C and E-enriched Artemia (CE1 group), HUFA without vitamin (HUFA group) and non-enriched Artemia (control) in 3 replicates. Enriched Artemia feeding for all treatments was initialized on day 17 and finished on day 23 post hatch. After the period of enrichment, the larvae were fed with Coppens diet from day 23 to 36 and then, the sampling was done. Larvae resistance to osmotic and temperature stresses was performed with submersion in fresh water (0.5-1 ppt) and cold water (15°C) for 1h, respectively. Larvae growth factors except for DGR were not significantly different between the groups (p>0.05). Fish fed non-enriched Artemia had significantly higher DGR (0.63 ± 0.04) (p<0.05). Mortality rate was significantly different between control and other groups from day 17 (p<0.05). Stress tolerance, cortisol and total protein were not significantly different among the groups. Glucose was only significantly different between C3 and CE2. Results indicate that enrichment of Artemia fransiscana with HUFA and vitamins E and C increases survival in yellowfin seabream larvae at its first feeding. © 2012 Adloo MN, et al.