MicroRNA‑100 suppresses human gastric cancer cell proliferation by targeting CXCR7

Abstract

microRNAs (miRs) are a class of small non‑coding RNAs that have been demonstrated to have a crucial role in tumorigenesis of human cancers, including gastric cancer (GC). Previous results have established that miR‑100 participated in the development of GC; however, the underlying mechanism remains largely unknown. The preesent study utilized reverse transcription‑quantitative polymerase chain reaction to analyze the expression of miR‑100 in GC tissues and adjacent normal tissues. The present results indicated that the expression of miR‑100 was downregulated in GC tissues when compared to the adjacent normal tissues. Furthermore, low miR‑100 expression was observed to be associated with lymph node metastasis, tumor diameter and tumor stage. In addition, Kaplan‑Meier analysis revealed that patients with low miR‑100 expression tended to have a shorter overall survival. The miR‑100 was further identified as an independent prognostic factor for overall survival. Notably, the levels of chemokine (CXC motif) receptor 7 (CXCR7) were inversely correlated with miR‑100 in GC cell lines. Furthermore, miR‑100 overexpression or CXCR7 depletion decreased in vitro GC cell proliferation. Bioinformatics analysis indicated that miR‑100 may bind to the 3’‑untranslated region of CXCR7 to prevent the initiation of protein translation. Thus, miR‑100 may function as a tumor suppressor in GC, partly by regulating the expression of CXCR7, and the regulation of miR‑100 expression may be a potential strategy for the treatment of GC patients.