An Improved Method for Perforated Patch Recordings Using Nystatin-Fluorescein Mixture

Abstract

This paper describes an improved method for solubilizing nystatin in an aqueous solution without the aid of organic solvents. The patch pipette was filled with a solution of nystatin and fluorescein sodium mixed in a molar ratio of 1:10. The success rate of perforated patch recordings was substantially improved, and the access resistance of 20-40 MΩ was readily achieved. © 1993, PHYSIOLOGICAL SOCIETY OF JAPAN. All rights reserved.