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Long non-coding RNA depletion using self-cleaving ribozymes

Humana Press Inc., (2021), 287-301
DOI: 10.1007/978-1-0716-0716-9_16
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Abstract

Some long non-coding RNA (lncRNA) genes encode a functional RNA product, whereas others act as DNA elements or via the act of transcription. We describe here a ribozyme-based approach to deplete an endogenous lncRNA in mouse embryonic stem cells, with minimal disruption of its gene. This enables the role of the lncRNA product to be tested.

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Tuck, A. C., & Bühler, M. (2021). Long non-coding RNA depletion using self-cleaving ribozymes. In Methods in Molecular Biology (Vol. 2167, pp. 287–301). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0716-9_16

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