Formulation, antioxidant and antibacteria activities of peel-off gel mask, enriched with bidara leaf (Ziziphus Spina-Christi L.) extract

Abstract

Bidara Leaves (Ziziphus spina-christi L.) is the one of the herbs that already known by people to repair the broken cell and acne's problem. The bidara leaves extract can be formulated into a peel-off gel mask for facial skin. The objective of this research is to formulate the peel-off gel mask from ethanol extract of bidara leaves. The peel-off gel mask expected effective in reducing the adverse effects of free radicals and inhibits the growth of acne-causing bacteria on the face. Identification of bioactive compounds in bidara leaves extract with phytochemical test and Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) instrument. Antioxidant activity test using 1,1-diphenyl-2-picrylhydrazil (DPPH) method and IC50 value determination. Testing of antibacterial activity of ethanol extract of bidara leaf against Propionibacterium acne bacteria by diffusion method. Preparation of gel mask peel-off using variation of concentration 15, 25 and 35%. Peel-off gel mask characterization by organoleptic test, physical appearance, pH value, homogeneity test, dispersion test, drying time test and microbial contamination. Ethanol extract of bidara leaves has very strong antioxidant activity with IC50 23,4 ppm and antibacterial activity with inhibition of bacteria P. acne at a concentration ≥ 0.25 g/mL The results of LC-MS/MS analysis showed that there were routine compounds at retention time of 5.13 which had the potential as antioxidants and antibacterial. Peel-off gel masks obtained have antioxidant and antibacterial activity and are included in the quality requirements category of SNI 16-6070-1999 and SNI 16-4380-1996.