Antigen-T Lymphocyte Interactions: Inhibition by Cytochalasin B

Abstract

The adsorption of cytologically active C57BL/6 T lymphocytes onto homologous DBA/2 embryonic fibroblast monolayers has been studied in the presence of a number of drugs. The adsorption of cytolytic effector cells onto monolayers was unaffected by prostaglandin E1 or by colchicine, both of which are potent inhibitors of lymphocyte-mediated cytolysis. On the other hand, the specific adsorption of lymphocytes onto the monolayers was markedly inhibited by cytochalasin B. These findings reinforce the concept that T cell-mediated cytolysis proceeds in discrete steps which are inhibitable at different levels. Further, in view of the known effects of cytochalasin B, these studies suggest that membrane modulation is required for T lymphocyte-antigen interactions.