Unique dietary-related mouse model of colitis

43Citations
Citations of this article
30Readers
Mendeley users who have this article in their library.

Abstract

Background: A high-fat diet is a risk factor for the development of inflammatory bowel disease (IBD) in humans. Deoxycholate (DOC) is increased in the colonic contents in response to a high-fat diet. Thus, an elevated level of DOC in the colonic lumen may play a role in the natural course of development of IBD. Methods: Wild-type B6.129 mice were fed an AIN-93G diet, either supplemented with 0.2% DOC or unsupplemented and sacrificed at 1 week, 1 month, 3 months, 4 months, and 8 months. Colon samples were assessed by histopathological, immunohistochemical, and cDNA microarray analyses. Results: Mice fed the DOC-supplemented diet developed focal areas of colonic inflammation associated with increases in angiogenesis, nitrosative stress, DNA/RNA damage, and proliferation. Genes that play a central role in inflammation and angiogenesis and other related processes such as epithelial barrier function, oxidative stress, apoptosis, cell proliferation/cell cycle/DNA repair, membrane transport, and the ubiquitin-proteasome pathway showed altered expression in the DOC-fed mice compared with the control mice. Changes in expression of individual genes (increases or reductions) correlated over time. These changes were greatest 1 month after the start of DOC feeding. Conclusions: The results suggest that exposure of the colonic mucosa to DOC may be a key etiologic factor in IBD. The DOC-fed mouse model may reflect the natural course of development of colitis/IBD in humans, and thus may be useful for determining new preventive strategies and lifestyle changes in affected individuals. Copyright © 2006 by Lippincott Williams & Wilkins.

References Powered by Scopus

Inflammatory bowel disease

3326Citations
N/AReaders
Get full text

Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice

1273Citations
N/AReaders
Get full text

Mucosal flora in inflammatory bowel disease

1182Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Carcinogenicity of deoxycholate, a secondary bile acid

297Citations
N/AReaders
Get full text

Bile acids as endogenous etiologic agents in gastrointestinal cancer

257Citations
N/AReaders
Get full text

Immune regulation by microbiome metabolites

256Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Bernstein, H., Holubec, H., Bernstein, C., Ignatenko, N., Gerner, E., Dvorak, K., … Payne, C. M. (2006). Unique dietary-related mouse model of colitis. Inflammatory Bowel Diseases, 12(4), 278–293. https://doi.org/10.1097/01.MIB.0000209789.14114.63

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 15

75%

Researcher 3

15%

Professor / Associate Prof. 2

10%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 9

43%

Medicine and Dentistry 7

33%

Biochemistry, Genetics and Molecular Bi... 3

14%

Nursing and Health Professions 2

10%

Save time finding and organizing research with Mendeley

Sign up for free