Undergraduate research in a biotechnology teaching laboratory: Expression and purification of a tyrosine kinase

Abstract

The class consisted of senior molecular biology majors who had previously taken cell biology, microbiology, biochemistry and molecular biology lecture courses but who had little or no previous lab experience. These students were asked to design and create an expression vector and purify the expressed protein. This project provides the students with the opportunity to appreciate interconnections between experiments while learning the necessary techniques.