While large amount of strains can be quickly generated via metabolic engineering, the speed/efficiency of evaluating each strain becomes the bottleneck in the process from strain development to final production. In this chapter, a method is introduced to rapidly evaluate strain performance in fed-batch fermentation mode by using dynamic dissolved oxygen stat feed back control with no additional advanced online measurement. In addition, a scale-down feature is integrated in the method to mimic the limitation of oxygen transfer in large-scale vessels, so that strains can be evaluated under the conditions close to that in large-scale bioreactors. The method has been implemented in several commercial standard benchtop scale fermentation systems with different fermentation control software. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Sun, J. (2012). Rapid strain evaluation using dynamic do-stat fed-batch fermentation under scale-down conditions. Methods in Molecular Biology, 834, 233–244. https://doi.org/10.1007/978-1-61779-483-4_15
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