Importance of schistosoma mansoni thioredoxin glutathione reductase: 1- evaluation of its role in schistosomiasis diagnosis in human

Abstract

Schistosoma parasite continued to be the focus of solicitude investigators to control the prevalence of the parasite. Efforts are needed for sensitive and accurate diagnosis that can be utilized to rapidly map the prevalence of the disease. The study aimed to benefit from the necessity of the S. mansoni for the enzyme Thioredoxin/Glutathione Reductase (TGR), in utilization of the enzyme as target antigen and to evaluate its efficacy in the diagnosis of S. mansoni infection patients. For reach the our goal, affinity chromatographic method has been utilized for isolation of TGR antigen from S. mansoni adult worm and employ this antigen for production of pAb for schistosomiasis by immunized rabbits. The pAb used in the efficacy evaluation of the purified pAb of TGR antigen in the diagnosis of S. mansoni in serum and urine of infected individual by indirect ELISA. The study, introduced a high diagnostic efficiency for the sandwich ELISA for detection of SmTGR antigen when applied to individuals with low or high worm burden in both serum and urine (89.73 and 87.02%, respectively). Sandwich ELISA revealed that this antigen can be relied on as a diagnostic target antigen in serum and urine.