Neuropeptides are important signaling molecules that regulate many essential physiological processes. Microdialysis offers a way to sample neuropeptides in vivo. When combined with liquid chromatography-mass spectrometry detection, many known and unknown neuropeptides can be identified from a live organism. This chapter describes sample preparation techniques and general strategies for the mass spectral analysis of neuropeptides collected via microdialysis sampling. Methods for the in vitro microdialysis of a neuropeptide standard as well as the in vivo microdialysis sampling of neuropeptides from a live crab are described.
CITATION STYLE
Behrens, H. L., & Li, L. (2010). Monitoring neuropeptides in vivo via microdialysis and mass spectrometry. Methods in Molecular Biology (Clifton, N.J.), 615, 57–73. https://doi.org/10.1007/978-1-60761-535-4_5
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