Genetic diversity among age classes of a Pinus sylvestris (L.) population from the Białowieza Primeval Forest, Poland

Abstract

The Białowieza Primeval Forest (BPF) is Europe's last primeval forest and an irreplaceable area for biodiversity conservation due to its size, protection status, and substantially undisturbed nature. There is no other forest in Europe with such a large surface representing highly-advanced natural succession. This article reports on the first analysis of the genetic variability and demographic structure of a self-renewed Pinus sylvestris population located in BPF, using both chloroplast and mitochondrial DNA markers. The analysis of molecular variance (AMOVA) for chloroplast simple sequence repeats (cpSSRs) revealed a significant genetic differentiation among age classes that accounted for about 2% of the total variance, comparable to those reported among different populations of Scots pine. None of the 117 detected chloroplast haplotypes were common to all age classes. Haplotype diversity ranged from 0.370 to 0.415 for cpSSRs and from 0.320 to 0.455 for mitochondrial markers. The genetic variation of the studied age classes-represented by mitochondrial markers-strongly depicts the maternal genetic structure, indicating limited seed dispersal. Temporal genetic substructuring is maintained within a self-renewed population of Scots pine from the BPF.