Lake trout Salvelinus namaycush on thiamine-replete and thiamine-depleted diets were evaluated for the effects of thiamine status on in vivo responses to theT-dependent antigen trinitophenol (TNP)-keyhole limpet hemocyanin (TNPKLH), the T-independent antigen trinitrophenol-lipolysaccaharide (TNP-LPS), or Dulbecco's phosphate-buffered saline (DPBS; negative control fish). Plasma antibody concentrations were evaluated for possible differences in total anti-TNP activity as well as differences in response kinetics. Associations between anti-TNP activity and muscle and liver thiamine concentrations as well as ratios of muscle-to-liver thiamine to anti-TNP activity were also examined. Thiamine-depleted lake trout that were injected with TNP-LPS exhibited significantly more anti-TNP activity than thiamine-replete fish. The depleted fish injected with TNP-LPS also exhibited significantly different response kinetics relative to thiamine-replete lake trout. No differences in activity or kinetics were observed between the thiaminereplete and -depleted fish injected with TNP-KLH or in the DPBS negative controls. Anti-TNP activity in thiaminedepleted lake trout injected with TNP-KLH was positively associated with muscle thiamine pyrophosphate (thiamine diphosphate; TPP) concentration. A negative association was observed between the ratio of muscle-to-liver TPP and T-independent responses. No significant associations between anti-TNP activity and tissue thiamine concentration were observed in the thiamine-replete fish. We demonstrated that thiamine deficiency leads to alterations in both T-dependent and T-independent immune responses in lake trout. © American Fisheries Society 2012.
CITATION STYLE
Ottinger, C. A., Honeyfield, D. C., Densmore, C. L., & Iwanowicz, L. R. (2012). Impact of thiamine deficiency on T-cell dependent and T-cell independent antibody production in lake trout. Journal of Aquatic Animal Health, 24(4), 258–273. https://doi.org/10.1080/08997659.2012.713890
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