Response surface methodology analysis of biodegradation of acrylonitrile in bioreactor

Abstract

Response surface methodology (RSM) was used to study the biodegradation effect of the various parameters namely, initial acrylonitrile(AN) concentration, pH, temperature and to optimize the process conditions for the maximum degradation of acrylonitrile by implementing the Box-Behnken statistical design in bioreactor. The analysis of variance (ANOVA) of the quadratic model demonstrates that the model was highly significant. Using this methodology, the optimal values of pH, temperature, initial AN concentration were 6.54, 26.22°C and 424.32mg/L, respectively. The experiment results showed that the RSM based on Box-Behnken design was useful for optimizing the biodegradation process. © 2011 Springer-Verlag.