DNA methylation is a major epigenetic modification that regulates gene expression, genome imprinting, and development and has a role in diseases including cancer. There are various methods for whole-genome methylation profiling that differ in cost and resolution. Recent advances in high-throughput sequencing technologies coupled with bisulfite treatment enable absolute DNA methylation quantification and genome-wide single-nucleotide resolution analysis. In this chapter, we provide detailed protocols for whole-genome bisulfite sequencing (WGBS), which captures the complete methylome. Using WGBS, we are able to generate a reference DNA methylome for normal or malignant hematopoietic cells.
CITATION STYLE
Jeong, M., Guzman, A. G., & Goodell, M. A. (2017). Genome-wide analysis of DNA methylation in hematopoietic cells: DNA methylation analysis by WGBS. In Methods in Molecular Biology (Vol. 1633, pp. 137–149). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7142-8_9
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