High-level production of Fc-fused kringle domain in Pichia pastoris

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Abstract

Recently, as a new non-immunoglobulin-based protein scaffold, a human kringle domain was successfully engineered toward biologically functional agonists and antagonists. In this study, the fed-batch cultivation conditions were optimized for enhanced production of an Fc-fused kringle domain (KD548-Fc) in Pichia pastoris. Fed-batch cultivations were performed in 5-l laboratory-scale bioreactors, and in order to find the optimal conditions for high-level production of KD548-Fc, several parameters including the initial carbon source (glycerol) concentration, temperature, and pH were investigated. When cells were cultivated at pH 4.0 and 25°C with 9.5% glycerol in the initial medium, the highest production yield (635 mg/l) was achieved with high productivity (7.2 mg/l/h). Furthermore, functional KD548-Fc was successfully purified from the culture broth using a simple purification procedure with high purity and recovery yield. © Society for Industrial Microbiology and Biotechnology 2014.

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APA

Jeong, G. M., Lee, Y. J., Kim, Y. S., & Jeong, K. J. (2014). High-level production of Fc-fused kringle domain in Pichia pastoris. Journal of Industrial Microbiology and Biotechnology, 41(6), 989–996. https://doi.org/10.1007/s10295-014-1435-2

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