Recent technological developments have enabled the characterization of the epigenetic landscape of single cells across a range of tissues in normal and diseased states and under various biological and chemical perturbations. While analysis of these profiles resembles methods from single-cell transcriptomic studies, unique challenges are associated with bioinformatics processing of single-cell epigenetic data, including a much larger (10–1,000×) feature set and significantly greater sparsity, requiring customized solutions. Here, we discuss the essentials of the computational methodology required for analyzing common single-cell epigenomic measurements for DNA methylation using bisulfite sequencing and open chromatin using ATAC-Seq.
CITATION STYLE
Lareau, C., Kangeyan, D., & Aryee, M. J. (2019). Preprocessing and computational analysis of single-cell epigenomic datasets. In Methods in Molecular Biology (Vol. 1935, pp. 187–202). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9057-3_13
Mendeley helps you to discover research relevant for your work.