Liposome Biodistribution via Europium Complexes

Abstract

Vector biodistribution is a requirement prior pharmaceutical development. Radioactive tracers allow the most sensitive and quantitative assessment of biodistribution, and conventional fluorophores are widely used in academic laboratories. We propose here to use europium complexes as a label for nanoparticles or biotherapeutics taking liposomes as models. Time-resolved fluorimetry (TRF) has the tremendous advantage of taking into accounts the fluorescence decay time of the lanthanide chelates, resulting in an improved sensitivity in biological media. The work described aimed following liposome biodistribution by TRF. An octadecyl-DTPA.Eu compound has been prepared and incorporated into liposomes without altering its fluorescence signal. The method has been validated through a comparison with fluorophore-labeled liposomes. The way to proceed when using this method for liposome biodistribution assessment is detailed. It could obviously be applied to other nanosystems, such as lipid nanoparticles.