Validação de métodos cromatográficos por clae para análise das vitaminas B1, B2, B6 e niacina naturalmente presentes em farinha de cereais

Abstract

o em 16/11/06; aceito em 27/9/07; publicado na web em 19/3/08 VALIDATION OF HPLC METHODS FOR ANALYSIS OF VITAMINS B1 , B2 , B6 AND NIACIN NATURALLY PRESENT IN CEREAL FLOURS. Complex B vitamins are present in some cereal foods and the ingestion of enriched products contributes to the recommended dietary intake of these micronutrients. To adapt the label of some products, it is necessary to develop and validate the analytical methods. These methods must be reliable and with enough sensitivity to analyze complex B vitamins naturally present in food at low concentration. The purpose of this work is to evaluate, with validated methods, the content of vitamins B1 , B2 and niacin in five cereal flours used in food industry (oat, rice, barley, corn and wheat).