Partial Purification of an Antidiarrheic Factor in Modified Cottonseed Oil Soapstock for Milk Replacer Rations

Abstract

A commercially manufactured feed-grade oil, Marcol B-75, reported previously to be strongly antidiarrheic when added to calf milk replacer diets, was systematically partitioned by three selected fractionation procedures: distillation, solubility in hexane, and saponification. The nondistillable, hexane-insoluble, saponifiable fraction was sufficiently antidiarrheic to prevent scouring in calves on milk replacer diets over-fed 60 to 100% above normal. The smallest active fraction, comprising 3% by volume of the original oil, was effectively antidiarrheic when incorporated into a milk replacer diet at a level of 0.3% by weight. © 1967, American Dairy Science Association. All rights reserved.