Antibacterial Efficacy of Selected Enterococcus Strains Isolated from Traditional Rice Beverage “Handia”

Abstract

The present study was conducted to evaluate the antibacterial efficacy of four bacteriocin producing Enterococcus strains isolated from traditional rice beverage, sourced from different sites of Baripada, Odisha, India. The strains were tested against three Gram-positive bacteria viz. Bacillus subtilis, Staphylococcus aureus, S. epidermidis and Pseudomonas aeruginosa, Salmonella typhimurium, Vibrio cholerae by agar cup method. Surprisingly, it was noticed that most of the Gram-positive bacteria were inhibited by cell free supernatant of isolated strains. The results indicated that the tested Gram-positive bacteria found more sensitive than Gram-negative, and were inhibited. Upon verification, all the isolated strains are found Gram-positive coccus, have the ability to resist the physiological concentration of bile salt, grow at temperature 10 to 42 o and purify the bacteriocin produced from the strains.