The membrane-binding domain of the Rous sarcoma virus Gag protein

  • Verderame M
  • Nelle T
  • Wills J
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Abstract

The Gag protein of Rous sarcoma virus (RSV) can direct particle assembly and budding at the plasma membrane independently of the other virus-encoded products. A previous deletion analysis has suggested that the first 86 amino acids of RSV Gag constitute a large membrane-binding domain that is absolutely required for these processes. To test this hypothesis, we inserted these residues in place of the N-terminal membrane-binding domain of the pp60v-src, a transforming protein whose biological activity requires plasma membrane localization. The ability of the Src chimera to induce cellular transformation suggests that the RSV sequence indeed contains an independent, functional domain.

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CITATION STYLE

APA

Verderame, M. F., Nelle, T. D., & Wills, J. W. (1996). The membrane-binding domain of the Rous sarcoma virus Gag protein. Journal of Virology, 70(4), 2664–2668. https://doi.org/10.1128/jvi.70.4.2664-2668.1996

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