Ligand binding to cell surface receptors activates signaling pathways in normal and pathologic conditions, and internalized ligand-receptor complexes may continue to signal from endosomes. Accessibility of cell surface receptors and the central function of ligand-receptor binding in signal transduction make ligand binding a prime target for therapeutic agents. We describe a Gaussia luciferase complementation method for imaging ligand-receptor binding in cell-based assays and living mice. While we illustrate this imaging method for chemokine ligand CXCL12 and its receptors CXCR4 and CXCR7, this imaging strategy can be generalized to a large number of ligand-receptor interactions. © 2014 Springer Science+Business Media New York.
CITATION STYLE
Luker, K. E., & Luker, G. D. (2014). Split Gaussia luciferase for imaging ligand-receptor binding. Methods in Molecular Biology, 1098, 59–69. https://doi.org/10.1007/978-1-62703-718-1_5
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