Epigenetic interactions among three dTph1 transposons in two homologous chromosomes activate a new excision-repair mechanism in Petunia

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Abstract

Unstable anthocyanin3 (an3) alleles of petunia with insertions of the Activator/Dissociation-like transposon dTph1 fall into two classes that differ in their genetic behavior. Excision of the (single) dTph1 insertion from class 1 an3 alleles results in the formation of a footprint, similar to the 'classical' mechanism observed for excisions of maize and snapdragon transposons. By contrast, dTph1 excision and gap repair in class 2 an3 alleles occurs via a newly discovered mechanism that does not generate a footprint at the empty donor site. This novel mechanism depends on the presence of two additional dTph1 elements: one located in cis, 30 bp upstream of the an3 translation start in the same an3 allele, and a homologous copy, which is located in trans in the homologous an3 allele. Absence of the latter dTph1 element causes a heritable suppression of dTph1 excision-repair from the homologous an3 allele by the novel mechanism, which to some extent resembles paramutation. Thus, an epigenetic interaction among three dTph1 copies activates a novel recombination mechanism that eliminates a transposon insertion.

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Van Houwelingen, A., Souer, E., Mol, J., & Koes, R. (1999). Epigenetic interactions among three dTph1 transposons in two homologous chromosomes activate a new excision-repair mechanism in Petunia. Plant Cell, 11(7), 1319–1336. https://doi.org/10.1105/tpc.11.7.1319

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