Abstract
Over the last decade, the laboratory rat has matured as a genetic model organism. One of the most valuable additions to its genetic toolbox is the ability to inactivate genes of interest using N-ethyl-N-nitrosourea mutagenesis-based reverse genetics. The Gould lab (McArdle Laboratory for Cancer Research, University of Wisconsin – Madison, USA) and the Cuppen lab (Hubrecht Institute for Developmental Biology and Stem Cell Research, Utrecht, The Netherlands) have developed this technology for a variety of rat strains. This resulted in the identification and characterization of a number of novel rat models, including novel models for breast and colon cancer. This chapter describes the establishment of the rat knockout technology and the characterization of the rat knockout models for the tumor suppressor genes APC, BRCA1, BRCA2, and MSH6.
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CITATION STYLE
Smits, B. M. G., Cuppen, E., & Gould, M. N. (2012). Target-selected enu mutagenesis to develop cancer models in the rat. In Genetically Engineered Mice for Cancer Research: Design, Analysis, Pathways, Validation and Pre-Clinical Testing (pp. 113–131). Springer New York. https://doi.org/10.1007/978-0-387-69805-2_6
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