4-Phosphothiophen-2-yl alanine: A new 5-membered analogue of phosphotyrosine

Abstract

Polyclonal antibodies raised against 4-phosphothiophen-2-yl alanine 2a, a novel five-membered ring analogue of phosphotyrosine, showed high selectivity for phosphotyrosine and no cross-reactivity with other phosphorylated amino acids. Western blots showed that the polyclonal was similarly effective, but different in selectivity, to a commercially available monoclonal antibody. © 2014 The Royal Society of Chemistry.