Molecular cloning of novel cytochrome P450 1A genes from nine Japanese amphibian species

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Abstract

Novel cytochrome P450 1A (CYP1A) cDNA fragments were isolated from the liver of nine Japanese amphibian species using reverse transcription polymerase chain reaction (RT-PCR). Degenerate PCR primers were used to amplify 122-bp fragments of CYP1A cDNAs. Construction of a phylogenetic tree revealed that urodele and anuran amphibians formed two branches. Within the anuran species, three branches were formed: 1) Ranidae and Rhacophoridae, 2) Bufo japonicus formosus and Hyla japonica, and 3) Xenopus laevis. The cDNA nucleotide sequence of these CYP1A fragments showed identities ranging 72-98% (all), 72-78% (Anura vs. Urodela), 75 to 98% (Anura), 81% (Urodela), 74-80% (Xenopus laevis vs. nine Japanese amphibians).

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Nakayama, S. M. M., Tanaka-Ueno, T., Sakamoto, K. Q., Fujita, S., & Ishizuka, M. (2009). Molecular cloning of novel cytochrome P450 1A genes from nine Japanese amphibian species. Journal of Veterinary Medical Science, 71(10), 1407–1411. https://doi.org/10.1292/jvms.001407

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