Mass spectrometric identification of ligands selected from combinatorial libraries using gel filtration

60Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.
Get full text

Abstract

There is a constant search for a successful analytical methodology to provide high throughput screening of combinatorial libraries against biological targets for identification of active ligands. Solid-phase screening assays offer faster isolation and identification of active analytes compared to the solution-based iterative methods. On the other hand, shift of combinatorial research to the creation of soluble non-peptide libraries, and limitations associated with the heterogeneous assays, creates a demand for a breakthrough technology for rapid and efficient screening of combinatorial libraries in solution. We demonstrated the efficient and rapid approach for selecting active ligands from a combinatorial mixture with subsequent identification of compounds by mass spectrometry. The procedure involves the use of a biological target molecule to physically isolate the active component in a mixture on a size exclusion medium. Then the ligands are identified using a combined liquid chromatography/capillary electrophoresis/mass spectrometry system. As a model system we used serum albumin and small molecules with different affinities to the protein.

Cite

CITATION STYLE

APA

Dunayevskiy, Y. M., Lai, J. J., Quinn, C., Talley, F., & Vouros, P. (1997). Mass spectrometric identification of ligands selected from combinatorial libraries using gel filtration. Rapid Communications in Mass Spectrometry, 11(11), 1178–1184. https://doi.org/10.1002/(SICI)1097-0231(199707)11:11<1178::AID-RCM991>3.0.CO;2-H

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free