There is a constant search for a successful analytical methodology to provide high throughput screening of combinatorial libraries against biological targets for identification of active ligands. Solid-phase screening assays offer faster isolation and identification of active analytes compared to the solution-based iterative methods. On the other hand, shift of combinatorial research to the creation of soluble non-peptide libraries, and limitations associated with the heterogeneous assays, creates a demand for a breakthrough technology for rapid and efficient screening of combinatorial libraries in solution. We demonstrated the efficient and rapid approach for selecting active ligands from a combinatorial mixture with subsequent identification of compounds by mass spectrometry. The procedure involves the use of a biological target molecule to physically isolate the active component in a mixture on a size exclusion medium. Then the ligands are identified using a combined liquid chromatography/capillary electrophoresis/mass spectrometry system. As a model system we used serum albumin and small molecules with different affinities to the protein.
CITATION STYLE
Dunayevskiy, Y. M., Lai, J. J., Quinn, C., Talley, F., & Vouros, P. (1997). Mass spectrometric identification of ligands selected from combinatorial libraries using gel filtration. Rapid Communications in Mass Spectrometry, 11(11), 1178–1184. https://doi.org/10.1002/(SICI)1097-0231(199707)11:11<1178::AID-RCM991>3.0.CO;2-H
Mendeley helps you to discover research relevant for your work.