RNA-sequencing (RNA-Seq) using next-generation sequencing (NGS) technique is a powerful tool for simultaneous analysis of global transcripts from both vaccinia virus and host cell. Here, we describe an RNA-Seq method for analyzing the vaccinia virus transcriptome from virus-infected HeLa cells. We pay particular attention to vaccinia virus-specific aspects of sample preparation, sequencing, and data analyses, but our method could be modified to analyze transcriptomes of other cells or tissues infected with different poxviruses.
CITATION STYLE
Cao, S., Lin, Y., & Yang, Z. (2019). Vaccinia Virus Transcriptome Analysis by RNA Sequencing. In Methods in Molecular Biology (Vol. 2023, pp. 157–170). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9593-6_10
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