Biopotential of Silver Nanopeptides Synthesized From Protein Extracts of Padina

Abstract

The present study was conducted to observe the bio-reduction of silver ions through the peptide solution of selected Padina species viz., P. pavonica, P. tetrastomatica and P. gymnospora. The aqueous silver ions exposed to the protein extracts and the silver nanopeptides synthesis was confirmed by the color change of protein extracts. These environmentally benign silver nanopeptides were further confirmed by using UV-Vis spectroscopy and powder X-ray diffraction analysis. The antibacterial activity of the synthesized nanopeptides against four selected pathogens viz., Escherichia coli, Pseudomonas aeruginosa, Staphylococcus pyogenes and Morganella morganii was carried out. The UV-Vis spectroscopic analysis confirmed the silver nanopeptides formation with the peak at 429 and 435nm. The protein extracts of P. pavonica, P. tetrastomatica and P. gymnospora mediated silver particles showed eight peaks with corresponding lattice planes which confirms the AgNP’s and they are crystalline in nature. The results also show that silver nanopeptides have good antibacterial activity against different bacterial species in which the protein extract of P. pavonica and P. gymnospora mediated silver particles showed the maximum zone of inhibition against M. morgonii (17 mm). The protein extract of P. tetrastomatica mediated AgNP’s showed the bactericidal activity against M. morganii (17 mm) and S. pyogenes (17 mm). The present study confirmed that the capping of proteins with silver nanoparticles capping is capable of rendering antibacterial efficacy and hence has a great potential in the preparation of therapeutic drugs used against bacterial diseases.