Preparation of Defatted Mustard by Extraction with Supercritical Carbon Dioxide

Abstract

The preparation- of defatted mustard by extraction with supercritical carbon dioxide (SC-C02is described. Oil was not extracted from native mustard seeds but was extracted from compressed mustard seeds with SC-C02 at 300 atm and 40°C. When the extraction time and temperature were kept constant at 3 hr and 40°C, 80~90% of the oil in the compressed mustard seeds could be extracted with SC-C02 at more than 300 atm. The solubility of the mustard seed oil in SC-C02 at 300 atm and 40°C was estimated to be about 0.48%. The synigrin content and myrosinase activity in the defatted mustard seeds with SC-C02 at 300 atm and 40°C for 3 hr were comparable to those in native seeds. Thus, the extraction with SC-C02 made it possible to prepare defatted mustard of high quality without decreasing the synigrin content and myrosinase activity. © 1987, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.