ELISA and Copan Based Evaluation and Analysis of Antibiotic Residues in Cattle Milk in Qazvin, Iran

Abstract

Aims: Daily consumption of milk containing antibiotic residues has become a great public health concern. This study aimed to evaluate the antibiotic residues in cattle raw milk using Copan milk test and specific enzyme-linked immunosorbent (ELISA) kits. Materials & Methods: In this study, 92 milk samples were collected from Qazvin province, Iran in two seasons during 2019. All the samples were analyzed by the Copan milk test and competitive ELISA kits to evaluate gentamicin, tetracycline, tylosin, chloramphenicol, penicillin, and sulfonamide residues in milk. Findings: The results showed that 45 milk samples (48.91%) were positive for the presence of antibiotic residues. Samples collected in summer were significantly (p