Mass spectrometry-based quantitative proteomics can identify and quantify thousands of proteins in complex biological samples. Improved instrumentation, quantification strategies and data analysis tools now enable protein analysis on a genome-wide scale. Particularly, quantification based on stable isotope labeling with amino acids (SILAC) has emerged as a robust, reliable and simple method for accurate large-scale protein quantification. The spectrum of applications ranges from bacteria and eukaryotic cell culture systems to multicellular organisms. Here, we provide a step-by-step protocol on how to plan and perform large-scale quantitative proteome analysis using SILAC, from sample preparation to final data analysis. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Kirchner, M., & Selbach, M. (2012). In vivo quantitative proteome profiling: Planning and evaluation of SILAC experiments. Methods in Molecular Biology, 893, 175–199. https://doi.org/10.1007/978-1-61779-885-6_13
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