Cysteine Lipidation on a Peptide or Amino acid (CLipPA) technology provides a facile method for the lipidation of unprotected peptides containing a free thiol group by using a “click” radical-initiated thiol-ene reaction to effect addition to a vinyl ester. The methodology is highly versatile, leading to high conversion rates while maintaining excellent chemoselectivity and tolerance for a large variety of peptide substrates and functional groups. Herein we describe the simple general procedure for the synthesis of a focused library of bioactive S-lipidated antimicrobial peptides via late-stage derivatization using solution-phase CLipPA lipidation.
CITATION STYLE
Hermant, Y. O., Cameron, A. J., Harris, P. W. R., & Brimble, M. A. (2020). Synthesis of antimicrobial lipopeptides using the “CLipPA” thiol-ene reaction. In Methods in Molecular Biology (Vol. 2103, pp. 263–274). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0227-0_18
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