Moranoline (1-Deoxynojirimycin) Fermentation and Its Improvemer

Abstract

Moranoline (1-deoxynojirimycin) is a strong a-glucosidase inhibitor. A rapid screening method for isolation of moranoline-producing Streptomyces (oblate*agar plate method) was developed. A glucoamylase inhibitor was isolated from the culture filtrate of Streptomyces lavendulae GC-148, a mutant strain obtained from Streptomyces lavendulae MB-733, a soil isolate; its spectral data were identical with those of moranoline from Morus species. Increased moranoline production was achieved through media improvement and mutagenic treatments (ultraviolet irradiation and N-methyl-N'-nitro-N-nitrosoguanidine treatment): 27 ~ 33fold augmentation (150 to 4000 ~ 5000 /ig/ml) was obtained. © 1985, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.