Antioxidant activity evaluation involving hemoglobin- related free radical reactivity

Abstract

Two methods for the measurement of antioxidant capacity are described: one based on a chronometric variation of a hemoglobin ascorbate peroxidase assay and he other based on electron paramagnetic resonance (EPR) spectra collected upon alkaline treatment of ethanolic samples. The involved chemical mechanisms are discussed, alongside the most important benefi ts and shortcomings; the assays offer new qualitative and quantitative information on samples of biological as well as synthetic origin.