Spectrophotometric analysis of metabolic enzyme activity from homogenized tissues is a valuable method for investigating mitochondrial content and capacity. Enzyme activity is normally measured in single cuvette spectrophotometers, requiring a large sample volume and low throughput. Here, we describe microplate assays for high-throughput analysis of mitochondrial enzymes citrate synthase, β-hydroxyacyl CoA dehydrogenase, aconitase, and mitochondrial electron transport system (ETS) complexes I, II, III, and IV.
CITATION STYLE
Janssen, R. C., & Boyle, K. E. (2019). Microplate assays for spectrophotometric measurement of mitochondrial enzyme activity. In Methods in Molecular Biology (Vol. 1978, pp. 355–368). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9236-2_22
Mendeley helps you to discover research relevant for your work.