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Journal Article

5' and 3' RACE Method to Obtain Full-Length 5' and 3' Ends of Ciona robusta Macrophage Migration Inhibitory Factors Mif1 and Mif2 cDNA

Methods in molecular biology (Clifton, N.J.) (2020) 2080 223-235
DOI: 10.1007/978-1-4939-9936-1_20
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Abstract

The 5' and 3' RACE is a method to obtain full-length 5' and 3' ends of cDNA using known cDNA sequences from expressed sequence tags (ESTs), subtracted cDNA, differential display, or library screening. Here is described the identification of full-length 5' and 3' ends of Ciona robusta Mif1 and Mif2 cDNA by using 5' and 3' RACE method.

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Vizzini, A. (2020). 5’ and 3’ RACE Method to Obtain Full-Length 5’ and 3’ Ends of Ciona robusta Macrophage Migration Inhibitory Factors Mif1 and Mif2 cDNA. Methods in Molecular Biology (Clifton, N.J.), 2080, 223–235. https://doi.org/10.1007/978-1-4939-9936-1_20

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