The structural organization of macromolecules and their association in assemblies and organelles is key to understand cellular function. Super-resolution fluorescence microscopy has expanded our toolbox for examining such nanometer-scale cellular structures, by enabling positional mapping of proteins in situ. Here, we detail the workflow to build nanometer-scale maps focusing on two complementary super-resolution modalities: structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM).
CITATION STYLE
Mennella, V., & Liu, Z. (2022). Nanometer-Scale Molecular Mapping by Super-resolution Fluorescence Microscopy. In Methods in Molecular Biology (Vol. 2440, pp. 305–326). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2051-9_18
Mendeley helps you to discover research relevant for your work.