Effect of indigenous microbial consortium on bioleaching of arsenic from contaminated soil by Shewanella putrefaciens

Abstract

The effects of indigenous microbial consortium on removal of As from As-contaminated soil using an Fe(III)-reducing bacterium Shewanella putrefaciens were investigated under circumneutral pH condition. Sequential extraction of As revealed that more than 30% of As was associated with Fe(III)-(oxy)hydroxides in the soil. Bioleaching experiments were conducted anaerobically with a supply of lactate as a carbon source. The highest As removal efficiency (57.5%) was obtained when S. putrefaciens and indigenous bacterial consortium coexisted in the soil. S. putrefaciens and indigenous bacteria solely removed 30.1% and 16.4% of As from the soil, respectively. The combination of S. putrefaciens and indigenous bacteria led to a higher amount of labile As after microbial dissolution of Fe(III)-(oxy)hydroxides. After microbial treatment, soil quality represented by pH and organic content appeared to be preserved. The results indicated that the ecological and physiological understanding of the indigenous microbiome might be important for the efficient application of bioleaching technology to remove As from contaminated soils.