Determination of zofenopril and its active metabolite in human plasma using high-performance liquid chromatography combined with a triple-quadruple tandem mass spectrometer

0Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

A simple, selective and sensitive LC-MS-MS method has been developed and validated to simultaneously quantify zofenopril and its active metabolite zofenoprilat in human plasma, using diazepam as internal standard. 1,4-Dithiothreitol was used as a reducer to release and stabilize the thiol group of zofenoprilat from dimer and mixed forms with endogenous thiols in the treatment of plasma samples. After a liquid-liquid extraction with methyl tert-butyl ether under acidic conditions, the post-treatment samples were analyzed on an Agilent ZORBAX Eclipse XDB-C8 column interfaced with a triple-quadruple tandem mass spectrometer using positive electrospray ionization. A solution of methanol and 0.1% formic acid solution (85:15, v/v) was used as the isocratic mobile phase with a flow rate of 0.2 mL/min. The method was validated to demonstrate the specificity, lower limit of quantitation, accuracy and precision of measurements. The validated LC-MS-MS method has been successfully applied to study the pharmacokinetics of zofenopril calcium in healthy Chinese volunteers.

References Powered by Scopus

Preclinical profile of zofenopril: An angiotensin converting enzyme inhibitor with peculiar cardioprotective properties

59Citations
N/AReaders
Get full text

High-performance liquid chromatography-electronspray ionization mass spectrometry for determination of tiopronin in human plasma

37Citations
N/AReaders
Get full text

Determination of captopril in human plasma, using solid phase extraction and high-performance liquid chromatography, coupled to mass spectrometry: Application to bioequivalence study

37Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Tian, Y., Cao, J., Luo, L., Zhang, Z., & Ma, P. (2015). Determination of zofenopril and its active metabolite in human plasma using high-performance liquid chromatography combined with a triple-quadruple tandem mass spectrometer. Journal of Chromatographic Science, 53(2), 253–262. https://doi.org/10.1093/chromsci/bmu050

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 8

89%

Researcher 1

11%

Readers' Discipline

Tooltip

Medicine and Dentistry 4

44%

Pharmacology, Toxicology and Pharmaceut... 3

33%

Chemistry 2

22%

Save time finding and organizing research with Mendeley

Sign up for free