Mammalian somatic cell nuclear transfer (SCNT) is a technically and biologically challenging procedure inducing rapid reprogramming of the nucleus from the differentiated into the totipotent state in a few hours. This procedure was initially successfully accomplished in farm animals, then in rodents, and more recently in primates and in humans. Though ethical concerns regarding SCNT still exist, this procedure can be utilized to generate patient and disease-specific pluripotent embryonic stem cell lines, which carry a great promise in improving our understanding of major disease conditions and a hope for better therapies and regenerative medicine. In this section, we will survey the existing literature and describe how mouse SCNT is performed and the importance of donor cell treatment and cycle synchronization prior to SCNT.
CITATION STYLE
Shufaro, Y., & Reubinoff, B. E. (2016). Nuclear treatment and cell cycle synchronization for the purpose of mammalian and primate Somatic Cell Nuclear Transfer (SCNT). In Methods in Molecular Biology (Vol. 1524, pp. 289–298). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6603-5_18
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