Isolation and characterization of bovine intestinal subepithelial myofibroblasts

8Citations
Citations of this article
18Readers
Mendeley users who have this article in their library.

Abstract

Intestinal subepithelial myofibroblasts (ISMFs) are mesenchymal cells that exist under the epithelium of intestines. Primarily isolated ISMFs from rodents have been applied to experiments. However, due to the size of their intestines, the available cell number is limited. Thus, we attempted to isolate ISMFs from bovine colon as an alternative material. After detachment of smooth muscle and epithelial layers, colonic mucosa was explanted. After 2-week incubation, α-SMA (+) / vimentin (+) / desmin (-) ISMFs were harvested and applied for experiments. First we examined the effect of cell passage on morphology and proliferation activity of bovine ISMFs. Although 3rd and 7th passage bovine ISMFs did not exhibit any changes, 11th passage ISMFs showed rounded enlarged shape and lost proliferation potential. On the contrary, rat ISMFs displayed the above senescent changes at earlier passage (passage 4). In intracellular Ca2+ concentration measurement, bioactive substances (0.3 - 1 μM ATP, 0.1 - 1 μM serotonin, 10 - 100 nM endothelin-1, and 1 - 10 nM bradykinin) dose-dependently induced an increase in intracellular Ca 2+ concentration in bovine ISMFs (passage 3 and 7). However, at passage 11, impairment in intracellular Ca2+ responses was observed. Thus, bovine ISMFs might be a novel useful tool with long life span and good cellular responses to investigate physiological/pathophysiological roles of ISMFs. ©2010 The Japanese Pharmacological Society.

Cite

CITATION STYLE

APA

Iwanaga, K., Murata, T., Hori, M., & Ozaki, H. (2010). Isolation and characterization of bovine intestinal subepithelial myofibroblasts. Journal of Pharmacological Sciences, 112(1), 98–104. https://doi.org/10.1254/jphs.09258FP

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free