High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching

  • Lee D
  • Lee M
  • Kwak H
  • et al.
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Abstract

Optical diffraction tomography (ODT) enables the three-dimensional (3D) refractive index (RI) reconstruction. However, when the RI difference between a sample and a medium increases, the effects of light scattering become significant, preventing the acquisition of high-quality and accurate RI reconstructions. Herein, we present a method for high-fidelity ODT by introducing non-toxic RI matching media. Optimally reducing the RI contrast enhances the fidelity and accuracy of 3D RI reconstruction, enabling visualization of the morphology and intra-organization of live biological samples without producing toxic effects. We validate our method using various biological organisms, including C. albicans and C. elegans.

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Lee, D., Lee, M., Kwak, H., Kim, Y. S., Shim, J., Jung, J. H., … Park, Y. (2022). High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching. Biomedical Optics Express, 13(12), 6404. https://doi.org/10.1364/boe.465066

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