Optical diffraction tomography (ODT) enables the three-dimensional (3D) refractive index (RI) reconstruction. However, when the RI difference between a sample and a medium increases, the effects of light scattering become significant, preventing the acquisition of high-quality and accurate RI reconstructions. Herein, we present a method for high-fidelity ODT by introducing non-toxic RI matching media. Optimally reducing the RI contrast enhances the fidelity and accuracy of 3D RI reconstruction, enabling visualization of the morphology and intra-organization of live biological samples without producing toxic effects. We validate our method using various biological organisms, including C. albicans and C. elegans.
CITATION STYLE
Lee, D., Lee, M., Kwak, H., Kim, Y. S., Shim, J., Jung, J. H., … Park, Y. (2022). High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching. Biomedical Optics Express, 13(12), 6404. https://doi.org/10.1364/boe.465066
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