An enzyme-linked assay for the measurement of amyloglucosidase in commercial enzyme mixtures and crude culture filtrates is described. A method for the synthesis of the substrate employed, p-nitrophenyl β-D-maltoside, is also described. The substrate is used in the presence of saturating levels of β-glucosidase. With a range of Aspergillus sp. culture filtrates, an excellent correlation was found for values obtained with this assay and a conventional assay employing maltose as substrate with measurement of released glucose. © 1991 Science & Technology Letters.
CITATION STYLE
McCleary, B. V., Bouhet, F., & Driguez, H. (1991). Measurement of amyloglucosidase using P-nitrophenyl β-maltoside as substrate. Biotechnology Techniques, 5(4), 255–258. https://doi.org/10.1007/BF02438658
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