Imaging molecularly defined regions of chromatin in single living cells during transcriptional activation has the potential to provide new insight into gene regulatory mechanisms. Here, we describe a method for isolating cell lines with multi-copy arrays of reporter transgenes, which can be used for real-time high-resolution imaging of transcriptional activation dynamics in single cells. © Springer Science+Business Media, LLC 2013.
CITATION STYLE
Rafalska-Metcalf, I. U., & Janicki, S. M. (2013). Preparation of cell lines for single-cell analysis of transcriptional activation dynamics. Methods in Molecular Biology, 977, 249–258. https://doi.org/10.1007/978-1-62703-284-1_20
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