The molluscicidal effect of the stem extracts of tinospora crispa in controlling the golden apple snail pomacea canaliculata

Abstract

This study investigated the molluscicidal effect of the stem extracts of Tinospora crispa in controlling the golden apple snail Pomacea canaliculata. Extracts were prepared in four solvents, i.e., hexane, chloroform, methanol, and distilled water at three concentrations (1,000, 5,000, and 10,000 ppm) per solvent. The phytochemical contents of the extracts were qualitatively identified, and the lethal concentration (LC50) of the extracts for mollusicicidal potential was determined using the probit analysis. The effect of T. crispa extracts on the snail was monitored for three days and the snail mortality was recorded every 24 h. The stem extract prepared in methanol at 10,000 ppm showed the highest molluscicidal effect with a mortality of 80% at 72 h. Phytochemicals identified in the stem extracts included alkaloids, flavonoids, saponin, tannin, and terpenoids. Based on the probit analysis, stem extracts of T. crispa prepared in methanol showed the lowest LC50 value of 3,428 ppm for mollusicicidal potential and followed by extracts prepared in chloroform, hexane, and distilled water at 5,888, 14,771, and 14,993 ppm, respectively.