Comparative study of electrochemical biosensors based on highly efficient mesoporous ZrO2-Ag-G-SiO2and In2O3-G-SiO2for rapid recognition of E. coli O157:H7

Abstract

Here, we reported an innovative and electrochemical biosensor for the rapid detection of Escherichia coli O157:H7. We fabricated the mesoporous ZrO2-Ag-G-SiO2 (ZAGS) and In2O3-G-SiO2 (IGS) sensors, and cyclic voltammetry (CV) was employed to detect the bacteria. The development of these portable sensors addresses the challenges of conventional time-consuming and more expensive laboratory-based analyses. Hence, the biosensors were highly selective to detect E. coli. The sensor could recognize an individual E. coli cell in 1 μL of sample volume within 30 s. E. coli live cells tied down on sample nanoparticles worked toward the definite acquirement of E. coli. The high thickness of negative charge on the surface of E. coli cells effectively regulated the concentration of dominant part charge carriers in the mesoporous channel, allowing a continuous check of E. coli concentration in a known sample. The signal current decreased linearly, while the E. coli concentration increased from 1.0 × 101 to 1.0 × 1010 CFU/mL. ZAGS and IGS biosensors could detect E. coli in the range from 101 to 1010 CFU/mL. ZAGS and IGS biosensors in this investigation showed great specificity, reproducibility, stability, and selectivity and are expected to have a great impact on applications in the detection of foodborne pathogens.